The effect of fractionation of the essential oil from pinus syvestris on the synergistic and antagonistic medicinal activities

Authors

  • Awojide H. Shola Department of Pure and Applied Chemistry, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Nigeria.
  • Adegbite Aishat Olasunnbo Department of Pure and Applied Chemistry, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Nigeria.
  • Oyewole A. Kehinde Department of Chemical Engineering, Osun State University, Osogbo, Nigeria.
  • Adeyemo G. Abayomi Department of Pure and Applied Chemistry, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Nigeria
  • Fadunmade E. Olumide Department of Pure and Applied Chemistry, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Nigeria
  • Jacob Anifowose Adebanjo Department of Pure and Applied Chemistry, Faculty of Basic and Applied Sciences, Osun State University, Osogbo, Nigeria

Keywords:

Pinus sylvestris, Medicinal, Synergistic, Essential oil

Abstract

Crude and fractionated essential oils from the seed of Pinus sylvestris were analysed by gas chromatography-mass spectrometry (GC-MS) and was evaluated for their synergistic and antagonistic medicinal activities against E.coli, Klebsiella pneumonia and Bacillus cereus. Column Chromatographic revealed that highest yield was obtained from fraction P1. The GC-MS analysis revealed major chemical constituents like isoborneol, Fenchol, δ-terpineol, Terpine-1-ol and Diisooctyl phthalate across the samples. The phytochemicals obtained showed the present of alkaloids, phenolic acid, tannin, saponin, flavonoids, terpenoids and cardiac glycosides in all the samples except alkaloids that was absent in the crude essential oil. The values for antioxidant and the ABTS activities varied across the samples with fraction P3 having the lowest IC50 value in both assays. For the α-amylase assay, least value was recorded for fraction P2 while for α-glucosidase assay, fraction P4 recorded the least value. The crude essential oil with a MIC value of 6.20 mg/mL was most effective against E.coli, while fractions P1, P2 and P4 and the crude essential oil were most effective against K. pneumonia, and fraction P2 was most effective against B. cereus. All the fractions and crude essential oil showed antioxidant, antidiabetic, and antibacterial activities, the activities of the crude essential oil were synergic only in relation to antibacterial activity on E.coli, while all the fractions proved the antagonistic activity of some components in the crude essential oil.

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Published

2023-05-31

Issue

Vol. 2 No. 1 (2023)

Section

Articles